Wednesday, August 3, 2011
The fourth paper from my thesis, entitled "Dual-trap optical tweezers with real-time force clamp control", has just been published online by Review of Scientific Instruments: http://link.aip.org/link/doi/10.1063/1.3615309 Here's a video from the paper. We are holding on to two micron sized plastic spheres with laser-beams (shown in the video as green/cyan cross-hairs). The lower beam/trap is [...]
Maybe the best collection ever of IOM, or any class really, radio sailing videos available online: http://www.youtube.com/user/Kingg2855 Way to go "Kingg2855" !
I've continued to translate into C++ the old cam-experiments I wrote in C#. The kd-tree search for which triangles lie under the cutter seems to work, and the best way to visualize what is going on is through a video. Trying Vimeo for a change, to see if it's any better than youtube for these [...]
Sunday, September 27, 2009
Testing how 720p @ 30 fps recorded with the 500D looks on youtube: Unfortunately the blog theme calls for 450 pixels wide pictures and videos, so you'll have to click through to youtube to see the glorious HD http://www.youtube.com/watch?v=G7xq1h5KwuE
About 9 hours compressed into 38 seconds. 566 frames shot at 1 minute intervals from around 10:00 in the morning to 19:36 in the evening. Played back at 15 frames per second, which makes for a ~900x speedup. I first re-sized the jpegs to 1024 pixels wide and then used this matlab script to assemble [...]
I'm testing an EMCCD camera. This is a video of fluorescently labeled DNA through a 100x epi-fluorescence microscope. Or you can try a slightly better quality wmv-download (82 Mb) Once we've had time to practice some more, it should look much cooler, something like these DNA-curtains, or DNA-ejection from bacteriophage lambda. But it's a start. Also [...]
Jumpcut is closing, so I needed to move this video to youtube. This relates to my earlier posts here http://www.anderswallin.net/2007/12/mowing-tactics/ and here http://www.anderswallin.net/2007/06/an-emergent-spiral/ When I find time to work on this next, there are many ideas for improvements: How to specify only climb/conventional milling (allowing only the right or left side of the cutter to [...]
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Here a DNA-molecule is being stretched between two optically trapped polystyrene micron-sized beads. We're using an FPGA-based real-time controller for steering the upper trap. It's programmed with a PI-loop which aims to keep the force acting on the lower bead constant. Around 10s into the video we switch on the feedback-loop and we see the [...]
A ~48 000 base-pair long (ca 16 um) piece of DNA is stretched between two optically trapped ca 2 um diameter polystyrene beads. Bright-field real-time view through a 100x microscope. Scale-bar in microns on the right.
Tuesday, January 27, 2009
Were stretching a 13 kb DNA-construct between two optically trapped beads. This isn't a video of the experiment itself, but of some entangled beads we found in an old sample. The beads are 3 or 2 micron in diameter, and we hold on to the middle big bead with the optical trap while moving around [...]