Fluorescent DNA

Some very early testing of fluorescence imaging in our optical tweezers instrument. A 10 kb long piece of DNA (ca 3 um long when stretched) is held between two optically trapped microspheres. The DNA is coated with a fluorescent dye (SYBR-gold) which is exited by a 488 nm blue laser and the fluorescence signal is collected with a CCD camera looking through a narrow-band filter centered on the emission spectrum of SYBR-gold.

At around 1:10 in the video there's a double-tether (two DNA-molecules between the beads). We don't want that but there is not much that we can do about it, except discard the data. At the very end there's an image of QDots on the coverglass surface.

Links - October 15, 2009

9k jog

I haven't completely forgot to exercise, but the lack of daylight and cold temperatures make it, at least mentally, a bit harder to get out there and run.

9k_2009oct14

Garmin 405cx aboard an IOM

race1-2

The 405cx gps-watch is small and light enough to just tape to the aft-deck of an IOM. Today's event in Tampere had 12 races but I missed a few due to electrical problems. Races 1 and 2 above, and races 7-12 below. It's funny how google-maps and google-earth show permanent winter and ice in Tampere 🙂

race7-12

The raw-data (tampere_gpsdata.zip) is available in TCX format as well as Google-earth KML format from the garmin connect website.

Anyone have any software or matlab code to extract the instantaneous speed from this data and plot it? Might be useful for further CFD investigations or VPP-programs. Mostly light and shifting no1 rig the whole day. It will be interesting to compare with no2 and no3 rig in a real breeze.

Are there even smaller and lighter GPS-dataloggers? How long before we can have a GPS on every boat and watch playback of the race afterwards at home?

Links - October 1, 2009