Fluorescent DNA

Some very early testing of fluorescence imaging in our optical tweezers instrument. A 10 kb long piece of DNA (ca 3 um long when stretched) is held between two optically trapped microspheres. The DNA is coated with a fluorescent dye (SYBR-gold) which is exited by a 488 nm blue laser and the fluorescence signal is collected with a CCD camera looking through a narrow-band filter centered on the emission spectrum of SYBR-gold.

At around 1:10 in the video there's a double-tether (two DNA-molecules between the beads). We don't want that but there is not much that we can do about it, except discard the data. At the very end there's an image of QDots on the coverglass surface.